TRYPTONE SOYA YEAST EXTRACT AGAR, MODIFIED

Description

Tryptone Soya Yeast Extract Agar, Modified is recommended for the isolation and cultivation of L. monocytogenes and Yersinia from foods in accordance with FDA BAM, 1998. Food samples with suspected Listerial or Yersinial contamination are processed for the bacterial isolation using respective selective agars. Purification of isolated colonies on this medium is a mandatory step in the conventional analysis because isolated colonies on selective agar media may still be in contact with an invisible weak background of partially inhibited competitors. ISO Committee has also recommended this medium for confirmation of Listeria species. According to FDAs enrichment procedure for isolation of L. monocytogenes from dairy products, the sample to be tested is inoculated in enrichment broth and incubated at 30?C for 24-48 hours.