TRYPTONE SALT BROTH (ISO:1999 ISO/DIS 6887-1:2017)

Description

Tryptone Salt Broth is recommended by ISO Committee for preparation of specimens, stock suspensions and decimal dilutions used in various microbiological tests of food specimens. For ten-fold serial dilutions, dispense the diluents in volume necessary for the preparation of the decimal dilutions into test tubes or flasks in quantities such that after sterilization each tube or flask contains 9.0 ml. Transfer 1 ml of the initial suspension by means of a pipette into a tube containing 9 ml of sterile diluent at the appropriate temperature. For optimal precision, avoid any contact between the pipette containing the inoculum and the sterile diluent. Mix thoroughly to obtain dilutions until the appropriate number of microorganisms has been obtained.