TRYPTONE SALT AGAR, W/1% NaCl

Description

Members of the genus Vibrio are defined as Gram-negative, asporogenous rods that are straight or have a single, rigid curve. They are motile; most have a single polar flagellum, when grown in liquid medium. Different methods used for the confirmation of Vibrio species include physical, biochemical and serological assays. Tryptone salt agar w/ 1% NaCl is used for the growth of Vibrio sp. in accordance with FDA BAM for differentiation of El Tor and Classical biotypes. Blend the food sample to be analysed with Alkaline peptone water (APW) in appropriate ratio and incubate as per the recommendation by FDA BAM. Pure cultures can be isolated from APW by plating a loopful of the inoculum into TCBS agar. For biochemical and serological identification of Vibrio, colonies from crowded plates must be streaked to Tryptone salt agar, w/1% NaCl for purity. Incubate overnight at 35 ?2? C and proceed with identification using a single isolated colony for differentiation of Classic and El Tor biotypes. Further biochemical tests can also be done using colonies from this medium.