TMB (3,3',5,5' Tetramethylbenzidine)

Description

3,3,5,5-Tetramethylbenzidine TMB is a noncarcinogenic substitute Ames test negative for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 resulting in a yellow color , and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.