THIOL MEDIUM

Description

Thiol Medium is used for culturing microorganisms from body fluids and also other materials containing antibiotics like penicillin, streptomycin or sulphonamides. The efficacy of Thiol Medium to retain viability of Vibrio was initially described by Huddleson. The ability of Thiol Medium to neutralize antibacterials was demonstrated by Christensen. This media can also be used for the cultivation and maintenance of Haemophilus, Vibrio and Meningococci. 10 ml of Thiol Medium has capacity to nullify 100 units of penicillin and 1000 units of streptomycin supporting good growth of Staphylococci and other test organisms. Even dilute inocula of the test organisms can initiate and result in good growth within 24 hours. For testing, medium is prepared and tested with and without concentrations of 5, 100 and 1000 units of penicillin and 100, 1000 and 10,000 micrograms of streptomycin per 10 ml of tube. It is further inoculated with test organisms and incubated at 18 – 48 hours at 35-37?C