SMART-Seq Stranded Kit

Description

The SMART-Seq Stranded Kit is used to generate strand-specific RNA-seq libraries for Illumina sequencing from 11,000 sorted cells or 10 pg10 ng of purified total RNA. This kit incorporates Takara Bios SMART SwitchingMechanismat the 5 end ofRNATemplate technology and includes refinements to the SMARTer method for stranded RNA-seq that simplify the library preparation workflow and improve sequencing performance. In addition, contrary to theSMART-Seq v4 Ultra Low Input RNA Kit for SequencingandSMART-Seq HT Kit, reverse transcription is initiated using random priming instead of oligo dT priming, thus capturing the full transcriptome instead of only the polyadenylated fraction. The SMART-Seq Stranded Kit was specifically designed to deliver highly sensitive and reproducible data from single cells while keeping the workflow short and user friendly. The kit does not require additional rRNA removal methods or kits and produces sequencing libraries that retain strand-of-origin information. The integrated removal of cDNAs derived from rRNA-typically present in high abundance following cDNA synthesis from total RNA inputs-makes the workflow extremely sensitive, yielding data that is highly reproducible with low mapping to rRNA. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back