Rat liver tissue lysate - mitochondrial extract

Description

Product was previously marketed under the MitoSciences sub-brand. 2 mg of purified rat liver mitochondrial membranes resuspended in 400 mul of heart mitochondria resuspension buffer 10 mM Tris.HCl, pH 7.8, 0.2 M sucrose, 0.2 mM EDTA, 1 mM PMSF . 1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes it is recommended that 180 mul of the mitochondrial membranes supplied be solubilized by the addition of 20 mul of 10 % lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant.