Pyruvate Kinase Assay Kit

Description

Pyruvate Kinase Assay Kit ab83432 is a no-wash assay with one 15 minute incubation at 25C. In the assay, pyruvate kinase acts on PEP and ADP to generate pyruvate, which is then oxidized. Readout is on any colorimetric 570 nm or fluorometric Ex/Em 535/587 nm plate reader. - Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs. A Pyruvate Kinase Activity Assay Kit is used in the study and diagnosis of diseases where glycolytic dysfunction is involved, such as pyruvate kinase deficiency, a hereditary cause of hemolytic anemia, and various cancers, where altered PK activity supports tumor metabolism. Its also relevant in metabolic and liver disorders, neonatal anemia, and toxicological studies, helping assess cellular energy production and metabolic disruption.