pTRE-Cycle1 Vector

Description

pTRE-Cycle1 is a bidirectional, Tet-inducible expression vector that allows you to reversibly regulate i.e., cycle the amount of a protein of interest in mammalian cells while coexpressing a second protein that is not cycled. Expression of both proteins is tightly regulated by the bidirectional, TRE-based promoter, PTight-Bi.Expression of the first gene of interest is controlled by an additional mechanism, since the resulting protein is fused to an N-terminal ProteoTunerTMdestabilization domain DD , which causes rapid proteasomal degradation of any protein to which it is fused. The degradation process can be reversed repeatedly by adding the stabilizing ligand Shield1 and then removing it from the culture medium. The second gene of interest, which is cloned into a separate multiple cloning site, will be coexpressed with the tagged protein, but will not acquire a DD-tag, and thus is subject only to Tet-based regulation. The vector is intended for use with any Tet-On or Tet-Off Advanced Expression System. To select stable cell lines, the vector must be cotransfected with one of the provided linear selection markers. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Inducible expression of two proteins of interest, one with a DD tag and one induced by tetracycline.pTRE-Cycle2 and pTRE-Cycle3 allow you to inducibly coexpress a red or green fluorescent protein along with your DD-tagged protein of interest. Back Independent inducible expression of two proteins in HEK 293 cells is shown for red and green fluorescent proteins Panel A and also for Sox2 and Oct4 Panel B .