pRetroQ-DsRed Monomer-C1 Vector

Description

The pRetroQ-DsRed Monomer-C1 vector is a high-titer, self-inactivating retroviral expression vector that is optimized to eliminate promoter interference from the upstream LTR in the integrated provirus. This vector expresses a monomeric mutant of theDiscosomasp. red fluorescent protein DsRed . pRetroQ-DsRed Monomer-C1 allows cloning of genes into the multiple cloning site MCS downstream of the DsRed-Monomer coding sequence, which are subsequently expressed as fusions to the C-terminus of the DsRed-monomer protein. Together, this vector allows the expression of fluorescent fusion proteins in difficult-to-transfect cells. It also contains a puromycin selection marker for selection of stable integrants. The unmodified vector will express DsRed-Monomer protein and may be used to produce marker virus to optimize infection protocols. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Back Retro-X retroviral vector maps.IRES-containing bicistronic retroviral vectors allow you to express two target genes Vector A: pQCXIX or a target gene and an antibiotic resistance gene Vector B: pQCXIH,pQCXIN and pQCXIP . Fluorescent-fusion retroviral vectors express your protein of interest fused to a fluorescent protein Vector C: pRetroQ-AcGFP1 N1/C1 and pRetroQ-DsRed Monomer N1/C1 . Knockout RNAi-Ready pSIREN-RetroQ retroviral vectors are prelinearized and ready to accept a dsDNA oligonucleotide encoding a shRNA of your choice, Vectors DandE: RNAi-Ready pSIREN-RetroQ-DsRedExpress or pSIREN-RetroQ-ZsGreen and RNAi-Ready pSIREN-RetroQ . To express shRNA under tight, inducible control, use the knockout Tet series of retroviral vectors Vector F: Knockout RNAi-Ready pSIREN-RetroQ-TetH and pSIREN-RetroQ TetP .