pPAmCherry-Mem Vector

Description

pPAmCherry-Mem Vector is mammalian expression vector encoding a fusion protein of PAmCherry and the N-terminal 20 amino acids of neuromodulin GAP-43 . PAmCherry is a photoactivatable mutant of the fluorescent protein mCherry. PAmCherry is non-fluorescence until photoactivated by a short exposure to light at a wavelength between 350 nm and 400 nm. The excitation/emission wavelengths of photoactivated PAmCherry are 564 nm and 595 nm. The GAP-43 fragment contains a signal for posttranslational palmitoylation of cysteins 3 and 4 that targets the fusion protein to the plasma membrane. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back The mCherry monoclonal antibody detects PAmCherry-N1 in the lysate of mammalian cells.HEK 293 cells were transiently transfected with pPAmCherry-N1. Cell lysates corresponding to 30,000 cells were prepared from HEK 293 cells transiently expressing PAmCherry-N1 Lane 1 or a negative control untransfected cells; Lane 2 . Both lysates and a positive control 5 ng recombinant mCherry; Lane 3 were separated by SDS-PAGE and analyzed by Western blot using the mCherry monoclonal antibody at the recommended dilution of 1:1000. Back Back