pmCherry-C1 Vector

Description

pmCherry-C1 is a mammalian expression vector designed to express a protein of interest fused to the C-terminus of mCherry, a mutant fluorescent protein derived from the tetramericDiscosomasp. red fluorescent protein, DsRed. The unmodified vector can be used to express mCherry in mammalian cells. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Schematic for fluorescent fusion protein vectors.Panel A.N-terminal fluorescent protein FP vector.Panel B.C-terminal fluorescent protein vector. Back mCherry-fusion constructs.HeLa cells were transiently transfected, via a lipid-based method, with mammalian expression vectors encoding mCherry fused to either human cytoplasmic actin Panel A or tubulin Panel B . Cells were fixed using 4 % paraformaldehyde and imaged 36 hr posttransfection with a 40X objective on a Zeiss Axioskop microscope using the 575/50, 610, 640/50 filter set. Back