pLVX-tdTomato-C1 Vector

Description

This lentiviral expression vector encodes the tdTomato fluorescent protein tag. This very bright red fluorescent protein is a genetic fusion of two copies of dTomato, which was specifically designed for low aggregation. It adopts an intramolecular tandem dimer structure that contributes to its exceptional brightness, yet it behaves like a monomer. Inserting a cDNA in the MCS downstream of the tdTomato coding sequence joins your protein of interest to the C-terminus of the tag, and allows the fusion protein to be tracked and studied in transduced cells. To package the vector into high-titer, replication-incompetent lentivirus, we recommend using Lenti-X Packaging Single Shots and the Lenti-X 293T Cell Line. The resulting lentivirus can then be used to transduce virtually any mammalian cell type. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Lentiviral vectors with fluorescent proteins.Lenti-X vectors contain sequence elements that facilitate lentiviral packaging and/or boost transgene expression, including the LTRs, packaging signal , Rev response element RRE , and central polypurine tract/central termination sequence cPPT/CTS from HIV-1; and the woodchuck hepatitis virus post-transcriptional regulatory element WPRE . Vectors can express your protein fused at its N- or C- terminus to either a green AcGFP1 or red DsRed-Monomer fluorescent protein tag, or coexpress it as a separate protein along with ZsGreen1 shown , mCherry, or tdTomato.