Phalloidin-iFluor 680 Reagent

Description

Phalloidin-iFluor 680 Reagent ab176760 is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. Phallodin-iFluor 680 can be detected with a fluorescent microscope at Ex/Em = 683/700 nm NIR range . Phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized. Review our other popular phalloidin dye conjugates, including Phalloidin-iFluor 488 ab176753, Phalloidin-iFluor 647, ab176759, Phalloidin-iFluor 594 ab176757, Phalloidin-iFluor 555 ab176756, and Rhodamine Phalloidin ab235138, or search our website to see the rest of the range. Staining fixed cell or tissue samples with phalloidin conjugates is very simple; it requires a single 20-90 min incubation with the phalloidin, followed by 3 short wash steps. Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step. When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin filaments, essentially stabilizing actin filaments through the prevention of filament depolymerisation.