OneScript Plus cDNA Synthesis Kit

Description

OneScript Plus cDNA Synthesis Kit contains a Moloney-Murine Leukemia Virus Reverse Transcriptase with genetic modifications to abolish RNase H activity to achieve thermal stability. This special mutant enzyme offers higher cDNA yields, longer cDNA up to 12 kb, and is able to perform under high temperatures 50C - 55C , facilitating the elimination of secondary structures associated with GC-rich RNA templates. OneScript Plus is formulated with abms RNaseOFF Ribonuclease Inhibitor, offering improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitors. RNaseOFF is stable even under very low concentrations of DTT < 1 mM , making it the best choice for ultimate RNA protection. Product Features Maximal flexibility in priming oligo dT , random primers, or gene-specific primers Robust cDNA synthesis from any RNA template High reproducibility and excellent yield Employs abms OneScript Plus Reverse Transcriptase Cat. No. G237 Included in abms PCR Buffet Program Product Component Quantity 5X RT Buffer 400 mul Oligo dT 10 muM 100 mul Random Primers 10 muM 100 mul dNTP 10 mM 100 mul Nuclease-Free H2O 2 x 1.0 ml OneScript Plus Reverse Transcriptase 100 rxn 100 mul ISO 13485:2016 MDSAP Certified Our PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek a MDSAP recognized auditing organization . This product is equivalent to RScript Plus cDNA Synthesis Kit Cat. No. G236EU .