NucleoSpin Gel & PCR Clean-up Midi

Description

The NucleoSpin Gel & PCR Clean-up Midi kit includes spin columns, collection tubes, and buffers for purification of DNA from PCR reactions or agarose gels. The kit enables recovery of DNA fragments ranging in size from 50 bp20 kb, and can be used to process up to 4 ml of PCR reaction mixture or 4 g of agarose gel in one prep. The binding capacity of the midi spin column is 75 mug, and typical elution volumes range from 200400 mul. The kit employs a user-friendly workflow that enables processing of 6 samples in 25 minutes. Cat. # 740986.20 includes sufficient quantities of reagents and materials for performing 20 preps. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Complete primer removal down to 65 bp using NucleoSpin Gel and PCR Clean-up Midi.PCR products of 252, 164, 100, and 65 bp were spiked with primers and purified with NucleoSpin Gel and PCR Clean-up Midi. Elution was performed using 400 mul of elution buffer. Shown in the image are the samples prior to Lane 1 and after Lane 2 PCR purification on a 2 % TAE agarose gel. Back Gel extraction using NucleoSpin Gel and PCR Clean-up Midi.pGEM T Easy vectors containing two different inserts were digested using EcoRI and analyzed by electrophoresis. Digested inserts and vectors were cut out of the gel and purified with NucleoSpin Gel and PCR Clean-up Midi. Gel purification was analyzed on a 1 % TAE agarose gel. Samples in each lane are as follows: 1. pGEM T Easy with 645-bp insert prior to digestion2. pGEM T Easy vector backbone and 645-bp insert after digestion3. 645-bp insert after gel extraction4. pGEM T Easy vector backbone after gel extraction5. pGEM T Easy with 982-bp insert prior to digestion6. pGEM T Easy vector backbone and 982-bp insert after digestion7. 982-bp insert after gel extraction8. pGEM T Easy vector backbone after gel extraction