NucleoMag DNA/RNA Water

Description

The NucleoMag DNA/RNA Water kit employs magnetic bead technology to enable efficient isolation of microbial DNA and RNA from diverse air and water samples using manual or automated processing. The kit is compatible with a variety of filters and filtration systems, and can be used to process salt or fresh water samples ranging from turbid to clear. The kit protocol enables efficient removal of inhibitory compounds that might interfere with downstream assays while avoiding the use of reducing agents, and also allows for incorporation of a homogenization step using MN bead tubes for improved sample lysis. Purified DNA and RNA obtained with the kit are suitable for downstream applications such as qPCR and NGS. Cat. # 744220.1 includes sufficient quantities of reagents and materials for processing 1 x 96 samples. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Back Overview of NucleoMag DNA/RNA Water protocol for manual processing of 96 samples using NucleoMag SEP. Back Efficient detection of microbial DNA from different water and air samples.Various water samples and an air sample were filtered and DNA extracted with the NucleoMag DNA/RNA Water kit was analyzed by PCR. Microbial DNA could be efficiently measured for all of the samples, demonstrating the versatility of the NucleoMag DNA/RNA Water kit. Back Compatibility with different homogenization devices.Nucleic acids were extracted from spiked or unspiked tapwater samples with the inclusion of a homogenization step on either a Vortex Genie 2 device using the MN Bead Tube Holder or a dedicated instrument Retsch , and resulting yields were measured by qPCR. Both homogenization approaches yielded comparable results for each sample type, indicating the flexibility of the NucleoMag DNA/RNA Water kit in regards to its compatibility with the respective homogenization devices. Back Compatibility with different filtration systems.qPCR was performed with nucleic acids isolated from either round filters or a cartridge system using the NucleoMag DNA/RNA Water kit. Comparable CT values were obtained for each prep, demonstrating the versatility of the kit in providing efficient sample processing for each filtration system. Back Efficient removal of PCR inhibitors.Serial dilutions were performed on nucleic acids extracted from three different samples pondwater, seawater, and lakewater and the dilutions were analyzed by qPCR. The linearity of the inhibition plots indicates the absence of PCR inhibition for each sample. Back Automated processing on popular platforms.The NucleoMag DNA/RNA Water kit was used to purify nucleic acids from two different water samples using two different automation platforms, KingFisher magnetic rods and epMotion liquid handling , respectively. qPCR analysis of the purfied nucleic acids indicated that comparable yields were obtained from each sample, indicating the adaptability of the kit to both automation approaches.