NucleoBond HMW DNA

Description

The NucleoBond HMW DNA kit employs gravity flow filtration in combination with anion-exchange technology to enable efficient recovery of high molecular weight DNA up to 200 kbp in length from a diverse array of input material, including bacteria, yeast, plants, insects, blood, cultured cells, and mammalian tissues. The kit provides a gentler alternative to magnetic bead-based approaches which include extensive pipetting and mixing steps that threaten DNA integrity, and offers superior performance vs. liquid phase methods e.g., salting out which can be tedious and time consuming and tend to yield DNA of lower purity. The versatile kit protocol enables processing of 12 samples in about two hours, and can be combined with additional upstream and downstream processing steps, including enzymatic or mechanical sample lysis e.g., MN bead tubes and DNA concentration/desalinization e.g., NucleoSpin and NucleoSnap finishers . DNA purified with the kit is ideal for analysis by long-read sequencing technologies such as SMRT PacBio and Nanopore Oxford Nanopore , as well as long-range short-read methods such as Next GEM 10x Genomics . Cat. # 740160.2 includes enough reagents and materials to process two samples. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back