MUG MFC AGAR

Description

Faecal coliforms are organisms present in the gastrointestinal tract of warm blooded animals. Presence of faecal coliforms in water is an indication of faecal contamination. MFC Agar, formulated by Geldrich et al is recommended for the detection of faecal coliforms in water samples by the membrane filter technique. Escherichia coli is a member of the faecal coliform group, which possess the enzyme ?-glucuronidase. This enzyme specifically cleaves 4-Methylumbelliferyl-? -D-Glucuronide (MUG) to form a fluorescent end product 4-Methylumbelliferone. This end product fluoresces under long wave UV light (366 nm). MUG MFC Agar is MFC Agar with added MUG and is recommended for cultivating and enumerating faecal coliforms by the membrane filtration technique.