MUD SF BROTH BASE

Description

The normal habitat of faecal Streptococci is the gastrointestinal tract of warm-blooded animals. The Enterococcus group is a subgroup of the faecal Streptococci that includes E. faecalis, E. faecium, S. gallinarum and S. avium. The Enterococci are differentiated from other Streptococci by their ability to grow in high sodium chloride concentration i.e. 6.5% at pH 9.6 and at 10?C to 45?C. The Enterococci portion of the faecal Streptococcus group is a valuable bacterial indicator for determining the extent of faecal contamination of recreational surface waters. The multiple tube techniques are applicable primarily to raw and chlorinated waste-water and sediments and can be used for fresh and marine waters. MUD SF Broth is prepared as per the formula accepted by ISO committee under the specification ISO 7899-1:1998 for detection and enumeration of Enterococci in surface and waste water by miniaturized method (MPN). The diluted sample is inoculated in a row of microtitre plate wells containing dehydrated culture medium. Once the microtitre plate is inoculated, cover with disposable sterile adhesive tape and incubate the plate at 44?C for minimum of 36 hours and maximum 72 hours. Observe under UV light at 366 nm in the dark after an incubation period of 36 to72 hours. The presence of Enterococci is indicated by fluorescence resulting from the hydrolysis of MUD. The results are reported as Most Probable Number per 100 ml.