Mouse C5a (Complement Component 5a) ELISA Kit

Description

Synonym: C5a. This Mouse C5a (Complement Component 5a) ELISA Kit applies to the in vitro quantitative determination of Mouse C5a concentrations in serum, plasma and other biological fluids. Type:Sandwich, Assay time:3.25h, Detection type:Colormetric, Precision:CV<10%, Assay Range:1.56-100ng/mL, Sensitivity:0.57ng/mL, Recovery:80%-120%, Cross reactivity:The kit detected Mouse C5a in the samples and no significant cross-species reactions were found. Detection Principle:This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse C5a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse C5a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse C5a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse C5a. You can calculate the concentration of Mouse C5a in the samples by comparing the OD of the samples to the standard curve.