mCherry ELISA Kit

Description

mCherry ELISA Kit ab221829 is a rapid single-wash 90-min ELISA kit to measure Human IL-1 beta in cell culture extracts, cell culture media and cell culture supernatant. This SimpleStep ELISA sensitivity is 5.64 pg/mL. How the assay works Human IL-1 beta SimpleStep ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA protocol summary in the image section for further details. Assay Specificity This kit is designed for the quantification of mCherry. The standard protein in this kit is full-length mCherry. Spiked protein experiments were used to validate cell culture supernatant and cell extract sample types. Serum, plasma citrate , plasma EDTA , plasma heparin , urine, saliva, milk, CSF, and tissue extract samples have not been tested with this kit. 1,600 pg/mL and 400 pg/mL of recombinant YFP, GFP, and RFP were individually tested for cros reactivity. No cross reactivity was observed. 1,600 pg/mL of recombinant YFP, GFP, and RFP were individually tested for interference with 200 pg/mL of recombinant mCherry. No interference was observed, with an average recovery of 97 % of mCherry protein. mCherry ELISA Kit ab221829 protocol summary: 1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature 2. Wash 3. Add TMB development solution - incubate for 10 min 4. Add stop solution 5. Read the results on a plate reader at 450nm How other researchers are using mCherry ELISA Kit ab221829 mCherry ELISA Kit ab221829 has been used to study transgenic lines as a model for lysosomal processing for drug screening 1 , to study gene therapies for wet AMD using a recombinant virus 2 . References: 1 Olivier Devuyst et al. 2020, PMID: 32061435, 2 Daniel M Lipinski et al. 2018, PMID: 30082848 Save your precious samples Our 384-well mCherry ELISA Kit only requires a maxmum of 12.5muL Design your own immunoassay We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation - mCherry Antibody Pair - BSA and Azide free ab243999 - Anti-mCherry antibody EPR20579-246 - BSA and Azide free Capture ab244649 - Anti-mCherry antibody EPR20579-1 - BSA and Azide free Detector ab244910 Related and recommended products mCherry ELISA Kit ab221829 is commonly used to study gene expression and protein interactions. mCherry is a fluroscent protein used for labeling and visualizing cellular processes. Other ELISA kits for labeling and visualization include: - GFP ELISA kit ab171581 - SEAP Reporter Gene Assay Kit Luminescence ab133077 Primary antibodies for labeling and visualization include: - FITC Anti-GFP Antibody ab6662 This kit recognizes mCherry fluorescent protein from the organism Discosoma sp. mCherry is a general-purpose, red monomer, fluorescent protein with superior photostability. The mCherry fluorescent protein is a variant of the Discosoma red DsRed protein and is excited at wavelengths longer than 600 nm. REACH authorisation Abcam has not and does not intend to apply for the REACH Authorisation of customers uses of products that contain European Authorisation list Annex XIV substances. It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.