M-FILTER RINSE BROTH

Description

The membrane filter technique is highly reproducible, can be used to test relatively large sample volumes and usually yields numerical results more rapidly than the multiple tube fermentation procedure. The membrane filter technique is extremely useful in monitoring drinking water and a variety of natural waters. M-Filter Rinse Broth complies with the formulation as described in USP. After membrane filtration it is often necessary to rinse the membrane filter in order to remove residues of liquid sample materials. Use of this medium is recommended if the sample contains higher hydrocarbons or fats. The rinsing fluid protects the microorganisms retained by the filter from physiological shock, thus enabling microbes to grow rapidly. If the sample contains large quantities of carbohydrates or fats, additional quantities of polysorbate 80 upto 9.0 gm/litre can be added to the broth medium in accordance with USP recommendations. After filtering the liquid sample, rinse the filter 3 times with 100 ml portions of the M-Filter Rinse Broth, then complete the test in usual way.