LURIA BROTH BASE, MILLER’S MODIFICATION

Description

This medium is based on original Luria broth formula described by Miller for the growth and maintenance of E. coli strains used in molecular microbiology. Luria broth Base, Miller is a nutritionally rich medium recommended for growth of pure cultures of recombinant strains. E. coli is grown in late log phase in LB medium. Some plasmid vectors may replicate to high copy numbers without selective amplification. Some vectors do not replicate so freely, and need to be selectively amplified. Chloramphenicol can be added to inhibit host synthesis and as a result prevent replication of the bacterial chromosome. Luria Broth Base, Miller?s modification contains one tenth and one twentieth the sodium chloride level of the Lennox and Miller formulations of LB Agar respectively. This helps the user to select the optimal salt concentration for a specific strain. The medium may be aseptically supplemented with glucose, if desired.