Library Quantification Kit

Description

The Library Quantification Kit is used for rapid library quantification prior to Illumina next-generation sequencing. The kit contains all of the components needed to determine the amount of library DNA genomic, cDNA, etc. in a sample using qPCR. Quantification is accomplished by comparison of the threshold cycle Ct of the diluted sample with a standard curve derived from four calibrated DNA standards. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Lot-to-lot variation for DNA Standards.Quantification of three lots of pre-diluted DNA Standards 0.01, 0.1, 1, and 10 pM was performed by real-time PCR using Terra qPCR Direct TB Green Premix following the protocol in the Library Quantification Kit user manual. All reactions were performed in triplicate, and the resulting Ct SDM values for the triplicates were averaged. The variation between lots was <5 %. Back Back