Lenti-XTM Integration Site Analysis Kit

Description

The Lenti-X Integration Site Analysis Kit contains all the reagents needed for PCR-based identification of lentiviral vector integration sites in transduced cell clones. The first step is the construction of pools of adaptor-ligated genomic DNA fragments, referred to as libraries. These libraries can then be used for identifying genomic DNA sequences flanking the 5 end of the integrated provirus using long-distance PCR. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Flow chart of the Lenti-X integration site analysis protocol.The major steps include construction of three viral integration libraries, PCR amplification of genomic DNA from the libraries, and analysis of PCR products. The gel shows a typical result. Lane 1: HpaI library. Lane 2: DraI library. Lane 3: SspI library. Lane 4: No template control. Lane M: DNA size markers. AP: Adaptor primer. LSP: Lentivirus-specific primer. Back