Lambda Phosphatase: sc-200312A

Description

available in 100,000 units in 0.25 ml sc-200312 and 20,000 units in 0.05 ml sc-200312A sizes Bacteriophage I, recombinant E. coli Lambda Phosphatase can be used to release phosphate groups from phosphorylated serine, threonine and tyrosine residues in proteins; also active on phosphorylated histidine residues 1 mL of 10X Reaction buffer and 1 mL of 10X MnCl 2 included with each size Lambda Phosphatase Lambda PP is a Mn 2+ -dependent protein phosphatase with activity towards phosphorylated serine, threonine and tyrosine residues. The enzyme is inhibited by vanadate ions and can be heat inactivated at 65A C for 1 hour in the presence of 50 mM EDTA Purity: minimum 95 % SDS-PAGE with no detectable protease, DNase, or RNase activity supplied as a solution containing approximately 400,000 units of protein per ml in 50 mM Tris-HCl, pH 7.5, 0.1 mM EGTA, 0.01 % BRIJ 35 and 50 % glycerol Unit Definition: One unit will hydrolyze 1 nmole of p-nitophenyl phosphate per minute at 30A C and pH 7.5 See product citations 53