LACTOBACILLI BROTH (AOAC) *

Description

Lactobacilli Broth, AOAC was formulated by Loy and recommended by AOAC for preparing inocula of test bacteria used for Microbiological assay of Vitamin B. Stock cultures of Lactobacillus leichmanni ATCC 7830, Lactobacillus plantarum ATCC 8014, Lactobacillus casei ATCC 7469, Enterococcus hirae ATCC 8043 and other such B vitamin requiring strains are prepared by stab inoculation of sterile Lactobacillus Agar, AOAC and incubated for 18-24 hours at a constant temperature between 30-40?C. Lactobacilli Broth, AOAC is used for cultivation and preparation of inocula of the above mentioned stock cultures. Inoculum is prepared by inoculating these cultures in Lactobacillus Broth, AOAC and incubating at 35-37?C.