ioSkeletal Myocytes - Human iPSC derived cells

Description

Introducing the ioSkeletal Myocytes, generated from human induced pluripotent stem cells iPSCs using opti-ox, a precise cellular reprogramming technology. Human stem cells, within days, convert into consistent and reliable skeletal myocytes, providing a high-quality human model for research, disease modelling and HTS. ioSkeletal Myocytes demonstrate robust expression of key proteins of myofilaments Desmin, Titin, Troponin, Myosin Heavy Chain, Dystrophin , coupled with the transition from immature MYH3 and MYH8 to mature myosin heavy chain isoforms MYH1 in a time dependent manner. By Day 10 post revival, skeletal myocytes form striated multinucleated myocytes that contract in response to acetylcholine. Human skeletal myocytes are available at scale, easy to culture and ready for experiments within days, providing a reliable model for the study of muscle, neuromuscular and associated metabolic disorders. In partnership with bit.bio Karyotype: Normal Seeding Density: 100,000 cells/cm2 Seeding compatibility: 6-, 12-, 24- and 96-well compatible Quality control: Sterility, ICC and gene expression analysis Research applications: Muscle research, Neuromuscular junction Research, Metabolic research, Drug development, Genetic screening e.g. CRISPR screening , Contractions assays This product is subject to limited use licenses from iPS Academia Japan Inc, TET Systems GmbH, ERS Genomics Limited and Sigma-Aldrich Co. LLC and is developed with Bit Bio patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.