ioGlutamatergic Neurons WT (isogenic control) - Human iPSC derived cells

Description

Note that this is suitable for studies with WT Glutamatergic Neurons and also serves as an isogenic control for disease models HTT, other . ioGlutamatergic Neurons have been reprogrammed from human induced pluripotent stem cells iPSC using opti-ox, a precise reprogramming technology. Human stem cells, within days, convert into consistent, mature, functional glutamatergic neurons providing a high quality human model for the study of neurological activity and disease. ioGlutamatergic Neurons cultures consist mainly of glutamatergic neurons 80 % characterised by the expression of the glutamate transporter genes VGLUT1 and VGLUT2 see Figure 1 . The minor remaining fraction of the neuronal population express marker genes of cholinergic neurons. A bulk RNA-seq analysis shows that ioGlutamatergic Neurons have a rostral CNS identity and express the classical cortical maker genes FOXG1 and TBR1 data not shown . In partnership with bit.bio Karyotype: Normal Seeding Density: 30,000 cells/cm2 Seeding compatibility: 6-, 12-, 24-, 96- and 384-well compatible Quality control: ICC and gene expression analysis Research applications: Academic research, Drug development, Neurotoxicology, Genetic screening e.g. CRISPR screening . This product is subject to limited use licenses from iPS Academia Japan Inc, TET Systems GmbH, ERS Genomics Limited and Sigma-Aldrich Co. LLC and is developed with Bit Bio patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.