Immortalized Baby Mouse Kidney Epithelial Cells (iBMK)

Description

The Immortalized Baby Mouse Kidney Epithelial Cells iBMK, Clone W2 is derived by co-expression of adenovirus E1A and dominant-negative p53 p53DD . In addition to its non-tumorigenicity, this cell line maintains its primary phenotype and expresses epithelial-specific markers such as E-cadherin, cytokeratin 8 and 18. Together with Bax Knockdown iBMK T3024 , Bak Knockdown iBMK T3025 and Bax/Bak Double Knockdown iBMK T3026 , these cell lines form the ideal system for studying mechanisms regulating apoptosis. In the same way, when paired with Atg5 Knockdown iBMK T3027 and Atg7 Knockdown iBMK T3028 , they present an attractive model for investigating the relationship between autophagy and metabolism homeostasis. Western blot was used to confirm the presence of E1A and p53DD gene in the immortalized cell line.