
0850341 · 5 MG
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Description
MOPC 21 Ascites fluid, collected from Balb/c mice which carry plasmacytomas subcutaneously, are clarified by centrifugation and filtration. It is dialyzed, adjusted to yield at least 5 mg of immunoglobulin, then vialed and lyophilized. Clarified ascites are tested for immunoglobulin by immunoelectrophoresis using antisera specific for the particular type and isotype. Approximately 10 % of the total protein is immunoglobulin including trace levels of the hosts immunoglobulins , 80 % is albumin, and 10 % is complement and other proteins.