Human CCL4 knockout THP-1 cell lysate

Description

Knockout cell lysate achieved by CRISPR/Cas9. Treatments: Human CCL4 knockout THP-1 cell lysate - Brefeldin A 5 mug/ml, 6h Human wild-type THP-1 cell lysate - Brefeldin A 5 mug/ml, 6h Human CCL4 knockout THP-1 cell lysate - PMA 100 ng/ml, 56h , LPS 1 mug/ml, 24h and Brefeldin A 5 mug/ml, during the last 6h of LPS treatment Human wild-type THP-1 cell lysate - PMA 100 ng/ml, 56h , LPS 1 mug/ml, 24h and Brefeldin A 5 mug/ml, during the last 6h of LPS treatment REACH authorisation Abcam has not and does not intend to apply for the REACH Authorisation of customers uses of products that contain European Authorisation list Annex XIV substances. It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail ratio: 300:100:10 . This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared. User storage instructions: Lyophilizate may be stored at 4C. After reconstitution, store at -20C for short-term storage or -80C for long-term storage.