Flag-His-Tag Rapid Assay Kit

Description

The Flag-His-Tag Rapid Assay Kit is developed based on colloidal gold lateral flow technology and the double antibody sandwich immunoassay principle. The chromatography membrane is immobilized with mouse anti-Flag-Tag monoclonal antibody as the test line and goat anti-mouse IgG polyclonal antibody as the control line; the gold conjugate pad is coated with colloidal gold-labeled mouse anti-His-Tag monoclonal antibody. When a sample containing Flag-His-Tag fusion protein is added to the sample pad, it moves towards the absorbent pad by capillary action. The target protein in the sample first binds to the gold-labeled antibody to form a complex, which is then captured and enriched by the antibody on the test line, forming a visible purplish-red band. This product has a detection sensitivity of 4ng/mL. Within the range of 4ng/mL to 500ng/mL, the color intensity of the test line is positively correlated with the protein concentration; when the protein concentration exceeds 500ng/mL up to 10μg/mL, the test line still maintains high-intensity color development without significant weakening due to antigen excess.