DOYLE’S ENRICHMENT BROTH BASE

Description

Campylobacter have long been known as pathogens of animal but two species i.e. Campylobacter jejuni and Campylobacter coli are now among the commonest identified cause of enteritis in man. It was due to the former lack of selective culture medium for their isolation from faeces that their role as human pathogens was not recognized. Doyles Enrichment Broth Base is recommended by APHA for enrichment of Campylobacter species. Dekeyser et al reported the isolation of Campylobacter jejuni from the faeces of patients with diarrhoea and acute gastroenteritis using a filtration technique and a selective medium with antimicrobics to suppress the normal enteric flora. Skirrow reported a selective medium containing three antimicrobics. Blaser et al reported success in isolating Campylobacter jejuni with a medium containing four antimicrobics added to Brucella Agar supplemented with defibrinated sheep blood.