COOKED MEAT MEDIUM (R.C. MEDIUM) (COMPLETE SOLUBLE)

Description

Cooked Meat Medium was originally developed by Robertson for the cultivation of certain anaerobes isolated from wounds. The present formulation is a modification, also called as Chopped M-Medium, which supports the growth of many spore forming and non-spore forming strict anaerobes. It has the ability to initiate growth of bacteria from very small inocula and to maintain the viability of cultures over long period. Mixed cultures of bacteria survive in Cooked Meat Medium without displacing the slower-growing organisms. The products of growth do not rapidly destroy the inoculated organisms and therefore it is an excellent medium for the storage of aerobic and anaerobic organisms. It is used for cultivation and maintenance of Clostridia and for determining proteolytic activity of anaerobes. FDA has recommended this medium for enumeration and identification of Clostridium perfringens from foods. For best results, medium should be used on the day it is prepared, otherwise it should be boiled or steamed for a few minutes and allowed to cool without agitation and then inoculated. Inoculation should be made near the bottom of the tube in the meat particles for anaerobic cultures. Aerobes grow at the top whilst more anaerobic species grow deeper in the medium.