CHROMOGENIC L-MONOLISTERIA DIFFERENTIAL AGAR

Description

Chromogenic L-Mono Listeria Differential Agar is based on the formulation of Ottaviani and Agosti and is used for isolation and cultivation of Listeria monocytogene. It allows selective identification and differentiation of Listeria monocytogenes. Listeria monocytogenes is a gram-positive foodborne human pathogen responsible for serious infections in pregnant women that may ultimately result in abortion, stillbirth, birth of a child with neonatal listeriosis and meningitis or primary bacteremia in adults and juveniles. The pathogenicity of Listeria ivanovii for humans is uncertain. This medium is based on the specific chromogenic detection of ?-glucosidase activity, rhamnose fermentation and PIPLC activity. Listeria species hydrolyse the purified chromogenic substrate in the medium giving blue coloured colonies. Since ?-glucosidase activity is specific for Listeria species, other organisms cannot utilize the chromogenic substrate and therefore give white colonies. Differentiation between Listeria species is based on the property of rhamnose fermentation and PIPLC activity.