BRILLIANT GREEN BILE AGAR (BRILLIANT GREEN LACTOSE BILE AGAR) (VEG.)

Description

Brilliant Green Veg Agar is prepared by replacing peptic digest of animal tissue and oxgall by Veg peptone and synthetic detergent No. II respectively which makes the medium free of BSE/TSE risks. Brilliant Green Veg Agar is the modification of Brilliant Green Bile Agar originally formulated as solid medium by Nobel and Tonney for the direct plating of materials of sanitary importance for enumeration of coliform bacteria. This medium is useful in selectively isolating Salmonella species from other coliform bacteria. Colonies of coliform bacteria are deep red surrounded by a pink halo against blue background of the medium, while Salmonella species, which do not ferment lactose, produce colourless to light pink colonies. It is recommended that the medium be prepared just prior to use and when necessary to store the medium, it should be kept in dark. Medium is sensitive to light, particularly direct sunlight, which will exhibit a decrease in the productivity of the medium and also colour may change from deep blue to purple or red.