BOLTON BROTH BASE (ISO 10272-1:2017)

Description

Foods of animal origin are the primary vehicles of Campylobacter infections in humans. Unpasteurized milk has been by far the most commonly implicated vehicle in the foodborne outbreaks of Campylobacter jejuni enteritis. Campylobacters were originally classified within the genus Vibrio, but they differ from Vibrios in their DNA Base composition and their ability to grow under conditions of reduced oxygen tension. Selective media were originally designed to isolate Campylobacter jejuni from faeces, by use of a cocktail of antibiotics in a rich basal medium. Bolton Broth Base is formulated as per recommendations of ISO for the selective enrichment of Campylobacter species from foods. The media is made selective for Campylobacters by addition of the antibiotics cefoperazone, vancomycin, trimethoprim and amphotericin B. These antibiotics are added as freeze dried supplements.