BDTM DimerX DimerX I: Recombinant Soluble Dimeric Mouse H-2K[b]:Ig Fusion Protein

Description

Schematic representation of the MHC class I:Ig dimeric protein. Flow cytometric analysis of T cells using BD DimerX H-2K b :Ig. Mouse BD DimerX H-2K b :Ig was incubated with a 40-molar excess of a specific peptide SIY SIYRYYGL, shaded histograms or an irrelevant peptide OVA258-276 SIINFEKL, unshaded histograms at 4C for 24 hours. Peptide-loaded H-2K b :Ig was then used for the immunofluorescent staining of cloned 2C T cells using either protocol 2 left panel or protocol 3 right panel . PE-conjugated anti-mouse IgG, mAb A85-1 Cat. No. 550083 was used to label the purified H-2K b :Ig BD DimerX molecule. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Schematic representation of the MHC class I:Ig dimeric protein. Show More Flow cytometric analysis of T cells using BD DimerX H-2K b :Ig. Mouse BD DimerX H-2K b :Ig was incubated with a 40-molar excess of a specific peptide SIY SIYRYYGL, shaded histograms or an irrelevant peptide OVA258-276 SIINFEKL, unshaded histograms at 4C for 24 hours. Peptide-loaded H-2K b :Ig was then used for the immunofluorescent staining of cloned 2C T cells using either protocol 2 left panel or protocol 3 right panel . PE-conjugated anti-mouse IgG, mAb A85-1 Cat. No. 550083 was used to label the purified H-2K b :Ig BD DimerX molecule. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More Schematic representation of the MHC class I:Ig dimeric protein. Flow cytometric analysis of T cells using BD DimerX H-2K b :Ig. Mouse BD DimerX H-2K b :Ig was incubated with a 40-molar excess of a specific peptide SIY SIYRYYGL, shaded histograms or an irrelevant peptide OVA258-276 SIINFEKL, unshaded histograms at 4C for 24 hours. Peptide-loaded H-2K b :Ig was then used for the immunofluorescent staining of cloned 2C T cells using either protocol 2 left panel or protocol 3 right panel . PE-conjugated anti-mouse IgG, mAb A85-1 Cat. No. 550083 was used to label the purified H-2K b :Ig BD DimerX molecule. Flow cytometry was performed on a BD FACSCalibur flow cytometry system.