BDTM Cytometric Bead Array (CBA) Rat TNF Flex Set

Description

Figure 1. Example BD CBA Rat TNF Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analyzed using FCAP Array Software. Linearity: Cell culture supernatant and 1:4 diluted pooled rat serum were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample. Reproducibility: The inter-assay and intra-assay reproducibility were determined for the BD CBA Rat TNF Flex Set by evaluating ten replicates of three different sample levels inter-assay and two replicates of three different sample levels from four separate experiments intra-assay respectively. Recovery: Cell culture supernatant and pooled rat serum were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. Serum samples were diluted 1:4 before the protein was spiked into each. Serum used was commercially available pooled rat serum. Figure 1. Example BD CBA Rat TNF Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analyzed using FCAP Array Software. Show More Linearity: Cell culture supernatant and 1:4 diluted pooled rat serum were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample. Show More Reproducibility: The inter-assay and intra-assay reproducibility were determined for the BD CBA Rat TNF Flex Set by evaluating ten replicates of three different sample levels inter-assay and two replicates of three different sample levels from four separate experiments intra-assay respectively. Show More Recovery: Cell culture supernatant and pooled rat serum were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. Serum samples were diluted 1:4 before the protein was spiked into each. Serum used was commercially available pooled rat serum. Show More Figure 1. Example BD CBA Rat TNF Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analyzed using FCAP Array Software. Linearity: Cell culture supernatant and 1:4 diluted pooled rat serum were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample. Reproducibility: The inter-assay and intra-assay reproducibility were determined for the BD CBA Rat TNF Flex Set by evaluating ten replicates of three different sample levels inter-assay and two replicates of three different sample levels from four separate experiments intra-assay respectively. Recovery: Cell culture supernatant and pooled rat serum were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. Serum samples were diluted 1:4 before the protein was spiked into each. Serum used was commercially available pooled rat serum.