BDTM Cytometric Bead Array (CBA) Mouse Soluble CD62E (E-Selectin) Flex Set

Description

Figure 1. Example BD CBA Mouse Soluble CD62E E-Selectin Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analyzed using FCAP Array Software. Reproducibility: The intra-assay and inter-assay reproducibility were determined for the BD CBA Mouse Soluble CD62E E-Selectin Flex Set by evaluating ten replicates of three different sample levels intra-assay and three replicates of three different sample levels from four separate experiments inter-assay . Linearity: Cell culture supernatant and 1:4 diluted pooled mouse serum were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample. ND = Not Determined. Due to high levels of CD62E E-Selectin in serum and EDTA-plasma samples, linearity information could not be generated. Recovery: Cell culture supernatant and pooled mouse serum were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. Serum samples were diluted 1:4 before the protein was spiked into each. Serum used was commercially available pooled mouse serum. ND = Not Determined. Due to high levels of CD62E E-Selectin in serum and EDTA-plasma samples, recovery information could not be generated. Figure 1. Example BD CBA Mouse Soluble CD62E E-Selectin Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analyzed using FCAP Array Software. Show More Reproducibility: The intra-assay and inter-assay reproducibility were determined for the BD CBA Mouse Soluble CD62E E-Selectin Flex Set by evaluating ten replicates of three different sample levels intra-assay and three replicates of three different sample levels from four separate experiments inter-assay . Show More Linearity: Cell culture supernatant and 1:4 diluted pooled mouse serum were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample. ND = Not Determined. Due to high levels of CD62E E-Selectin in serum and EDTA-plasma samples, linearity information could not be generated. Show More Recovery: Cell culture supernatant and pooled mouse serum were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. Serum samples were diluted 1:4 before the protein was spiked into each. Serum used was commercially available pooled mouse serum. ND = Not Determined. Due to high levels of CD62E E-Selectin in serum and EDTA-plasma samples, recovery information could not be generated. Show More Figure 1. Example BD CBA Mouse Soluble CD62E E-Selectin Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analyzed using FCAP Array Software. Reproducibility: The intra-assay and inter-assay reproducibility were determined for the BD CBA Mouse Soluble CD62E E-Selectin Flex Set by evaluating ten replicates of three different sample levels intra-assay and three replicates of three different sample levels from four separate experiments inter-assay . Linearity: Cell culture supernatant and 1:4 diluted pooled mouse serum were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample. ND = Not Determined. Due to high levels of CD62E E-Selectin in serum and EDTA-plasma samples, linearity information could not be generated. Recovery: Cell culture supernatant and pooled mouse serum were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. Serum samples were diluted 1:4 before the protein was spiked into each. Serum used was commercially available pooled mouse serum. ND = Not Determined. Due to high levels of CD62E E-Selectin in serum and EDTA-plasma samples, recovery information could not be generated.