BDTM Cytometric Bead Array (CBA) Human Granzyme A Flex Set

Description

Figure 1. Example BD CBA Human Granzyme A Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analyzed using FCAP Array Software. Linearity: Cell culture supernatant, 1:4 diluted serum, or 1:4 diluted EDTA-treated plasma were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample. Reproducibility: The intra-assay and inter-assay reproducibility were determined for the BD CBA Human Granzyme A Flex Set by evaluating ten replicates of three different sample levels intra-assay and three replicates of three different sample levels from four separate experiments inter-assay . Recovery: Cell culture supernatant, serum, or EDTA-treated plasma were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. Serum and plasma samples were diluted 1:4 before the protein was spiked into each. Serum is a pool of 800 - 1000 donors and the plasma was pooled from at least 20 donors. The spike recovery for serum and plasma is influenced by the presence of antithrombin III ATIII and alpha-2-macroglobulin alpha2M , therefore this assay may not be useful for serum and plasma samples. Please see References for more information. Figure 1. Example BD CBA Human Granzyme A Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analyzed using FCAP Array Software. Show More Linearity: Cell culture supernatant, 1:4 diluted serum, or 1:4 diluted EDTA-treated plasma were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample. Show More Reproducibility: The intra-assay and inter-assay reproducibility were determined for the BD CBA Human Granzyme A Flex Set by evaluating ten replicates of three different sample levels intra-assay and three replicates of three different sample levels from four separate experiments inter-assay . Show More Recovery: Cell culture supernatant, serum, or EDTA-treated plasma were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. Serum and plasma samples were diluted 1:4 before the protein was spiked into each. Serum is a pool of 800 - 1000 donors and the plasma was pooled from at least 20 donors. The spike recovery for serum and plasma is influenced by the presence of antithrombin III ATIII and alpha-2-macroglobulin alpha2M , therefore this assay may not be useful for serum and plasma samples. Please see References for more information. Show More Figure 1. Example BD CBA Human Granzyme A Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analyzed using FCAP Array Software. Linearity: Cell culture supernatant, 1:4 diluted serum, or 1:4 diluted EDTA-treated plasma were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample. Reproducibility: The intra-assay and inter-assay reproducibility were determined for the BD CBA Human Granzyme A Flex Set by evaluating ten replicates of three different sample levels intra-assay and three replicates of three different sample levels from four separate experiments inter-assay . Recovery: Cell culture supernatant, serum, or EDTA-treated plasma were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. Serum and plasma samples were diluted 1:4 before the protein was spiked into each. Serum is a pool of 800 - 1000 donors and the plasma was pooled from at least 20 donors. The spike recovery for serum and plasma is influenced by the presence of antithrombin III ATIII and alpha-2-macroglobulin alpha2M , therefore this assay may not be useful for serum and plasma samples. Please see References for more information.