BD PhosflowTM PE Mouse anti-PDPK1 (pS241)

Description

Analysis of PDPK1 pS241 in human peripheral blood mononuclear cells PBMC . PBMC were either treated with calyculin A plus okadaic acid for 30 minutes at 37oC solid line histograms or untreated dashed line histograms . The cells were fixed with pre-warmed BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 15 minutes at 37oC and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes. The treated and untreated PBMC were stained with PE Mouse IgG1, kappa isotype control mAb MOPC-21 Cat. No. 559320, left panel or PE Mouse anti-PDPK1 pS241, Cat. No. 560092, right panel . The data demonstrates that the phosphorylation of PDPK1 is constitutive in all PBMC and that the level of phosphorylation increases when phosphatase activity is inhibited by the treatment. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Analysis of PDPK1 pS241 in human peripheral blood mononuclear cells PBMC . PBMC were either treated with calyculin A plus okadaic acid for 30 minutes at 37oC solid line histograms or untreated dashed line histograms . The cells were fixed with pre-warmed BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 15 minutes at 37oC and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes. The treated and untreated PBMC were stained with PE Mouse IgG1, kappa isotype control mAb MOPC-21 Cat. No. 559320, left panel or PE Mouse anti-PDPK1 pS241, Cat. No. 560092, right panel . The data demonstrates that the phosphorylation of PDPK1 is constitutive in all PBMC and that the level of phosphorylation increases when phosphatase activity is inhibited by the treatment. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Analysis of PDPK1 pS241 in human peripheral blood mononuclear cells PBMC . PBMC were either treated with calyculin A plus okadaic acid for 30 minutes at 37oC solid line histograms or untreated dashed line histograms . The cells were fixed with pre-warmed BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 15 minutes at 37oC and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes. The treated and untreated PBMC were stained with PE Mouse IgG1, kappa isotype control mAb MOPC-21 Cat. No. 559320, left panel or PE Mouse anti-PDPK1 pS241, Cat. No. 560092, right panel . The data demonstrates that the phosphorylation of PDPK1 is constitutive in all PBMC and that the level of phosphorylation increases when phosphatase activity is inhibited by the treatment. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More Analysis of PDPK1 pS241 in human peripheral blood mononuclear cells PBMC . PBMC were either treated with calyculin A plus okadaic acid for 30 minutes at 37oC solid line histograms or untreated dashed line histograms . The cells were fixed with pre-warmed BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 15 minutes at 37oC and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes. The treated and untreated PBMC were stained with PE Mouse IgG1, kappa isotype control mAb MOPC-21 Cat. No. 559320, left panel or PE Mouse anti-PDPK1 pS241, Cat. No. 560092, right panel . The data demonstrates that the phosphorylation of PDPK1 is constitutive in all PBMC and that the level of phosphorylation increases when phosphatase activity is inhibited by the treatment. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More Analysis of PDPK1 pS241 in human peripheral blood mononuclear cells PBMC . PBMC were either treated with calyculin A plus okadaic acid for 30 minutes at 37oC solid line histograms or untreated dashed line histograms . The cells were fixed with pre-warmed BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 15 minutes at 37oC and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes. The treated and untreated PBMC were stained with PE Mouse IgG1, kappa isotype control mAb MOPC-21 Cat. No. 559320, left panel or PE Mouse anti-PDPK1 pS241, Cat. No. 560092, right panel . The data demonstrates that the phosphorylation of PDPK1 is constitutive in all PBMC and that the level of phosphorylation increases when phosphatase activity is inhibited by the treatment. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Analysis of PDPK1 pS241 in human peripheral blood mononuclear cells PBMC . PBMC were either treated with calyculin A plus okadaic acid for 30 minutes at 37oC solid line histograms or untreated dashed line histograms . The cells were fixed with pre-warmed BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 15 minutes at 37oC and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes. The treated and untreated PBMC were stained with PE Mouse IgG1, kappa isotype control mAb MOPC-21 Cat. No. 559320, left panel or PE Mouse anti-PDPK1 pS241, Cat. No. 560092, right panel . The data demonstrates that the phosphorylation of PDPK1 is constitutive in all PBMC and that the level of phosphorylation increases when phosphatase activity is inhibited by the treatment. Flow cytometry was performed on a BD FACSCalibur flow cytometry system.