BD PhosflowTM Alexa Fluor 488 Mouse antic-Cbl (pY700)

Description

Analysis of c-Cbl pY700 in activated human T leukemia cells. Jurkat cells ATCC TIB152 were serum starved overnight and then either stimulated with 5 mM hydrogen peroxide for 15 minutes shaded histogram or unstimulated open histogram . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes, then permeabilized BD PhosFlow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 488 anti-c-Cbl pY700, Cat. No. 558101 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Analysis of c-Cbl pY700 in activated human T leukemia cells. Jurkat cells ATCC TIB152 were serum starved overnight and then either stimulated with 5 mM hydrogen peroxide for 15 minutes shaded histogram or unstimulated open histogram . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes, then permeabilized BD PhosFlow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 488 anti-c-Cbl pY700, Cat. No. 558101 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More Analysis of c-Cbl pY700 in activated human T leukemia cells. Jurkat cells ATCC TIB152 were serum starved overnight and then either stimulated with 5 mM hydrogen peroxide for 15 minutes shaded histogram or unstimulated open histogram . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes, then permeabilized BD PhosFlow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 488 anti-c-Cbl pY700, Cat. No. 558101 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system.