BD PhosflowTM Alexa Fluor 488 Mouse anti-PKCalpha

Description

Analysis of PKC alpha in lymphocytes. Human peripheral blood mononuclear cells PBMC were either stimulated with 10 muM Phorbol 12-myristate 13-acetate PMA, Sigma-Aldrich, Cat. No. P8139 for 24 hours shaded histogram or unstimulated open histogram, left figure . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 488 Mouse anti-PKCalpha Cat. No. 560242 . Lymphocytes were selected by scatter profile. The data demonstrates that the expression of PKCalpha decreases when the lymphocytes are stimulated by PMA. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. The specificity of mAb 3/PKC alpha was confirmed by western blot right figure analysis using Purified Mouse Anti-PKC alpha antibody Cat. No. 610107/610108 at 0.063 mug/ml on lysates from control lane 1 and PMA-treated lane 2 PBMC. PKC alpha is identified as a band of 82 kDa, which decreases in intensity in the treated cells. Purified Mouse anti-Actin monoclonal antibody Cat. No. 612656/612657 was the gel-loading control. Analysis of PKC alpha in lymphocytes. Human peripheral blood mononuclear cells PBMC were either stimulated with 10 muM Phorbol 12-myristate 13-acetate PMA, Sigma-Aldrich, Cat. No. P8139 for 24 hours shaded histogram or unstimulated open histogram, left figure . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 488 Mouse anti-PKCalpha Cat. No. 560242 . Lymphocytes were selected by scatter profile. The data demonstrates that the expression of PKCalpha decreases when the lymphocytes are stimulated by PMA. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. The specificity of mAb 3/PKC alpha was confirmed by western blot right figure analysis using Purified Mouse Anti-PKC alpha antibody Cat. No. 610107/610108 at 0.063 mug/ml on lysates from control lane 1 and PMA-treated lane 2 PBMC. PKC alpha is identified as a band of 82 kDa, which decreases in intensity in the treated cells. Purified Mouse anti-Actin monoclonal antibody Cat. No. 612656/612657 was the gel-loading control. Analysis of PKC alpha in lymphocytes. Human peripheral blood mononuclear cells PBMC were either stimulated with 10 muM Phorbol 12-myristate 13-acetate PMA, Sigma-Aldrich, Cat. No. P8139 for 24 hours shaded histogram or unstimulated open histogram, left figure . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 488 Mouse anti-PKCalpha Cat. No. 560242 . Lymphocytes were selected by scatter profile. The data demonstrates that the expression of PKCalpha decreases when the lymphocytes are stimulated by PMA. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. The specificity of mAb 3/PKC alpha was confirmed by western blot right figure analysis using Purified Mouse Anti-PKC alpha antibody Cat. No. 610107/610108 at 0.063 mug/ml on lysates from control lane 1 and PMA-treated lane 2 PBMC. PKC alpha is identified as a band of 82 kDa, which decreases in intensity in the treated cells. Purified Mouse anti-Actin monoclonal antibody Cat. No. 612656/612657 was the gel-loading control. Show More Analysis of PKC alpha in lymphocytes. Human peripheral blood mononuclear cells PBMC were either stimulated with 10 muM Phorbol 12-myristate 13-acetate PMA, Sigma-Aldrich, Cat. No. P8139 for 24 hours shaded histogram or unstimulated open histogram, left figure . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 488 Mouse anti-PKCalpha Cat. No. 560242 . Lymphocytes were selected by scatter profile. The data demonstrates that the expression of PKCalpha decreases when the lymphocytes are stimulated by PMA. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. The specificity of mAb 3/PKC alpha was confirmed by western blot right figure analysis using Purified Mouse Anti-PKC alpha antibody Cat. No. 610107/610108 at 0.063 mug/ml on lysates from control lane 1 and PMA-treated lane 2 PBMC. PKC alpha is identified as a band of 82 kDa, which decreases in intensity in the treated cells. Purified Mouse anti-Actin monoclonal antibody Cat. No. 612656/612657 was the gel-loading control. Show More Analysis of PKC alpha in lymphocytes. Human peripheral blood mononuclear cells PBMC were either stimulated with 10 muM Phorbol 12-myristate 13-acetate PMA, Sigma-Aldrich, Cat. No. P8139 for 24 hours shaded histogram or unstimulated open histogram, left figure . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 488 Mouse anti-PKCalpha Cat. No. 560242 . Lymphocytes were selected by scatter profile. The data demonstrates that the expression of PKCalpha decreases when the lymphocytes are stimulated by PMA. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. The specificity of mAb 3/PKC alpha was confirmed by western blot right figure analysis using Purified Mouse Anti-PKC alpha antibody Cat. No. 610107/610108 at 0.063 mug/ml on lysates from control lane 1 and PMA-treated lane 2 PBMC. PKC alpha is identified as a band of 82 kDa, which decreases in intensity in the treated cells. Purified Mouse anti-Actin monoclonal antibody Cat. No. 612656/612657 was the gel-loading control. Analysis of PKC alpha in lymphocytes. Human peripheral blood mononuclear cells PBMC were either stimulated with 10 muM Phorbol 12-myristate 13-acetate PMA, Sigma-Aldrich, Cat. No. P8139 for 24 hours shaded histogram or unstimulated open histogram, left figure . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 488 Mouse anti-PKCalpha Cat. No. 560242 . Lymphocytes were selected by scatter profile. The data demonstrates that the expression of PKCalpha decreases when the lymphocytes are stimulated by PMA. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. The specificity of mAb 3/PKC alpha was confirmed by western blot right figure analysis using Purified Mouse Anti-PKC alpha antibody Cat. No. 610107/610108 at 0.063 mug/ml on lysates from control lane 1 and PMA-treated lane 2 PBMC. PKC alpha is identified as a band of 82 kDa, which decreases in intensity in the treated cells. Purified Mouse anti-Actin monoclonal antibody Cat. No. 612656/612657 was the gel-loading control.