BD PharmingenTM Purified Rat Anti-Mouse TNF

Description

Immunocytochemical staining of TNF expression in mouse splenocytes. RBC-lysed BALB/c splenocytes were cultured with PMA Sigma, 5 ng/ml and ionomycin Sigma, 500 ng/ml with GolgiPlug Cat. No. 555029 for 4 hr at 37C. The activated cells were harvested and the presence of TNF producing cells was detected by immunocytochemistry using a three-step staining procedure that employs Biotin Goat anti-Rat IgG secondary antibody Cat. No. 559286 and Anti-Rat Ig HRP Detection Kit Cat. No. 551013 Nomarski optics, original magnification 400 X . To demonstrate the specificity of Purified Rat Anti-Mouse TNF Cat. No. 559064 , the antibody was blocked by the preincubation with excess recombinant mouse TNF Cat. No. 554589; data not shown . Immunocytochemical staining of TNF expression in mouse splenocytes. RBC-lysed BALB/c splenocytes were cultured with PMA Sigma, 5 ng/ml and ionomycin Sigma, 500 ng/ml with GolgiPlug Cat. No. 555029 for 4 hr at 37C. The activated cells were harvested and the presence of TNF producing cells was detected by immunocytochemistry using a three-step staining procedure that employs Biotin Goat anti-Rat IgG secondary antibody Cat. No. 559286 and Anti-Rat Ig HRP Detection Kit Cat. No. 551013 Nomarski optics, original magnification 400 X . To demonstrate the specificity of Purified Rat Anti-Mouse TNF Cat. No. 559064 , the antibody was blocked by the preincubation with excess recombinant mouse TNF Cat. No. 554589; data not shown . Show More Immunocytochemical staining of TNF expression in mouse splenocytes. RBC-lysed BALB/c splenocytes were cultured with PMA Sigma, 5 ng/ml and ionomycin Sigma, 500 ng/ml with GolgiPlug Cat. No. 555029 for 4 hr at 37C. The activated cells were harvested and the presence of TNF producing cells was detected by immunocytochemistry using a three-step staining procedure that employs Biotin Goat anti-Rat IgG secondary antibody Cat. No. 559286 and Anti-Rat Ig HRP Detection Kit Cat. No. 551013 Nomarski optics, original magnification 400 X . To demonstrate the specificity of Purified Rat Anti-Mouse TNF Cat. No. 559064 , the antibody was blocked by the preincubation with excess recombinant mouse TNF Cat. No. 554589; data not shown .