BD PharmingenTM Purified Rat Anti-Mouse CD124

Description

Expression of cell surface CD124 by normal splenocyres from BALB/c and C57BL/6 mice. Spleen cells from BALB/c left panel and C57BL/6 right panel mice were treated with ACK lysis buffer, were washed, and were labeled with purified Fc Block Cat. No. 553142; Rat IgG2b anti-mouse CD16/CD32 to block mouse Fc receptors. The cells were then stained with mIL4R-M1 1 mug followed by biotinylated RG7/1.30 mouse anti-rat IgG2alpha; 0.25 mug, Cat. No. 553894 and streptavidin phycoerythrin 0.015 mug, Cat. No. 554061 in a three-layer staining protocol to amplify immunofluorescent signals. Staining with the mIL4R-M1 antibody filled histograms is compared to staining with the secondary reagents alone open histograms . Expression of cell surface CD124 by normal splenocyres from BALB/c and C57BL/6 mice. Spleen cells from BALB/c left panel and C57BL/6 right panel mice were treated with ACK lysis buffer, were washed, and were labeled with purified Fc Block Cat. No. 553142; Rat IgG2b anti-mouse CD16/CD32 to block mouse Fc receptors. The cells were then stained with mIL4R-M1 1 mug followed by biotinylated RG7/1.30 mouse anti-rat IgG2alpha; 0.25 mug, Cat. No. 553894 and streptavidin phycoerythrin 0.015 mug, Cat. No. 554061 in a three-layer staining protocol to amplify immunofluorescent signals. Staining with the mIL4R-M1 antibody filled histograms is compared to staining with the secondary reagents alone open histograms . Expression of cell surface CD124 by normal splenocyres from BALB/c and C57BL/6 mice. Spleen cells from BALB/c left panel and C57BL/6 right panel mice were treated with ACK lysis buffer, were washed, and were labeled with purified Fc Block Cat. No. 553142; Rat IgG2b anti-mouse CD16/CD32 to block mouse Fc receptors. The cells were then stained with mIL4R-M1 1 mug followed by biotinylated RG7/1.30 mouse anti-rat IgG2alpha; 0.25 mug, Cat. No. 553894 and streptavidin phycoerythrin 0.015 mug, Cat. No. 554061 in a three-layer staining protocol to amplify immunofluorescent signals. Staining with the mIL4R-M1 antibody filled histograms is compared to staining with the secondary reagents alone open histograms . Show More Expression of cell surface CD124 by normal splenocyres from BALB/c and C57BL/6 mice. Spleen cells from BALB/c left panel and C57BL/6 right panel mice were treated with ACK lysis buffer, were washed, and were labeled with purified Fc Block Cat. No. 553142; Rat IgG2b anti-mouse CD16/CD32 to block mouse Fc receptors. The cells were then stained with mIL4R-M1 1 mug followed by biotinylated RG7/1.30 mouse anti-rat IgG2alpha; 0.25 mug, Cat. No. 553894 and streptavidin phycoerythrin 0.015 mug, Cat. No. 554061 in a three-layer staining protocol to amplify immunofluorescent signals. Staining with the mIL4R-M1 antibody filled histograms is compared to staining with the secondary reagents alone open histograms . Show More Expression of cell surface CD124 by normal splenocyres from BALB/c and C57BL/6 mice. Spleen cells from BALB/c left panel and C57BL/6 right panel mice were treated with ACK lysis buffer, were washed, and were labeled with purified Fc Block Cat. No. 553142; Rat IgG2b anti-mouse CD16/CD32 to block mouse Fc receptors. The cells were then stained with mIL4R-M1 1 mug followed by biotinylated RG7/1.30 mouse anti-rat IgG2alpha; 0.25 mug, Cat. No. 553894 and streptavidin phycoerythrin 0.015 mug, Cat. No. 554061 in a three-layer staining protocol to amplify immunofluorescent signals. Staining with the mIL4R-M1 antibody filled histograms is compared to staining with the secondary reagents alone open histograms . Expression of cell surface CD124 by normal splenocyres from BALB/c and C57BL/6 mice. Spleen cells from BALB/c left panel and C57BL/6 right panel mice were treated with ACK lysis buffer, were washed, and were labeled with purified Fc Block Cat. No. 553142; Rat IgG2b anti-mouse CD16/CD32 to block mouse Fc receptors. The cells were then stained with mIL4R-M1 1 mug followed by biotinylated RG7/1.30 mouse anti-rat IgG2alpha; 0.25 mug, Cat. No. 553894 and streptavidin phycoerythrin 0.015 mug, Cat. No. 554061 in a three-layer staining protocol to amplify immunofluorescent signals. Staining with the mIL4R-M1 antibody filled histograms is compared to staining with the secondary reagents alone open histograms .