BD PharmingenTM Purified Mouse anti-LAT (pY171)

Description

Western blot analysis of LAT pY171 on stimulated human T lymphocytes. Human Jurkat T cell leukemia; ATCC TIB-152 cells were either left untreated Left Panel or were activated with Purified NA/LE Mouse anti-Human CD3 cat No. 555329 and Purified NA/LE Mouse anti-Human CD28 Cat No. 555725 Right Panel and were then lysed. The lysates were then probed with Purified Mouse anti-LAT pY171 Cat. No. 558392 at concentrations of 2.0, 1.0, and 0.5 mug/ml Lanes 1, 2, and 3, respectively . LAT pY171 is identified as a band of 38 kDa in the treated cells. Western blot analysis of LAT pY171 on stimulated human T lymphocytes. Human Jurkat T cell leukemia; ATCC TIB-152 cells were either left untreated Left Panel or were activated with Purified NA/LE Mouse anti-Human CD3 cat No. 555329 and Purified NA/LE Mouse anti-Human CD28 Cat No. 555725 Right Panel and were then lysed. The lysates were then probed with Purified Mouse anti-LAT pY171 Cat. No. 558392 at concentrations of 2.0, 1.0, and 0.5 mug/ml Lanes 1, 2, and 3, respectively . LAT pY171 is identified as a band of 38 kDa in the treated cells. Show More Western blot analysis of LAT pY171 on stimulated human T lymphocytes. Human Jurkat T cell leukemia; ATCC TIB-152 cells were either left untreated Left Panel or were activated with Purified NA/LE Mouse anti-Human CD3 cat No. 555329 and Purified NA/LE Mouse anti-Human CD28 Cat No. 555725 Right Panel and were then lysed. The lysates were then probed with Purified Mouse anti-LAT pY171 Cat. No. 558392 at concentrations of 2.0, 1.0, and 0.5 mug/ml Lanes 1, 2, and 3, respectively . LAT pY171 is identified as a band of 38 kDa in the treated cells.