BD PharmingenTM Purified Mouse anti-Human SOX1

Description

Western Blot analysis of SOX1. Lysate from neural stem cells NSCs derived from H9 human ES cells WiCell, Madison, WI was probed with Purified Mouse anti-Human SOX1 monoclonal antibody at titrations of 0.5 lane 1 , 0.25 lane 2 , and 0.125 mug/ml lane 3 . SOX1 is identified as a tight doublet of almost 39 kDa. Immunofluorescent staining of Human SOX1. NSCs derived from H9 human ES cells WiCell Madison, WI were fixed, permeabilized with BD Perm/Wash Buffer Cat No. 554723 , and stained with purified anti-human SOX1 0.5 ug/test . The second step reagent was Alexa Fluor 488 goat anti-mouse Ig Life Technologies and counter-staining was with Hoechst 33342 pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD Attovision Software. Triton X-100 is also suitable for permeabilization. Western Blot analysis of SOX1. Lysate from neural stem cells NSCs derived from H9 human ES cells WiCell, Madison, WI was probed with Purified Mouse anti-Human SOX1 monoclonal antibody at titrations of 0.5 lane 1 , 0.25 lane 2 , and 0.125 mug/ml lane 3 . SOX1 is identified as a tight doublet of almost 39 kDa. Show More Immunofluorescent staining of Human SOX1. NSCs derived from H9 human ES cells WiCell Madison, WI were fixed, permeabilized with BD Perm/Wash Buffer Cat No. 554723 , and stained with purified anti-human SOX1 0.5 ug/test . The second step reagent was Alexa Fluor 488 goat anti-mouse Ig Life Technologies and counter-staining was with Hoechst 33342 pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD Attovision Software. Triton X-100 is also suitable for permeabilization. Show More Western Blot analysis of SOX1. Lysate from neural stem cells NSCs derived from H9 human ES cells WiCell, Madison, WI was probed with Purified Mouse anti-Human SOX1 monoclonal antibody at titrations of 0.5 lane 1 , 0.25 lane 2 , and 0.125 mug/ml lane 3 . SOX1 is identified as a tight doublet of almost 39 kDa. Immunofluorescent staining of Human SOX1. NSCs derived from H9 human ES cells WiCell Madison, WI were fixed, permeabilized with BD Perm/Wash Buffer Cat No. 554723 , and stained with purified anti-human SOX1 0.5 ug/test . The second step reagent was Alexa Fluor 488 goat anti-mouse Ig Life Technologies and counter-staining was with Hoechst 33342 pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD Attovision Software. Triton X-100 is also suitable for permeabilization.